羊附红细胞体病PCR检测方法的建立   总被引：5，自引：0，他引：5  

杨鹏华  秦建华  赵月兰  李玉文  张富梅 《中国兽医学报》2005,25(4):366-367

根据羊附红细胞体的16S rRNA基因参考序列，设计1对特异性引物．建立了检测羊附红细胞体的PCR技术。用本方法从感染血样中特异扩增出1条预期大小为1169bp的片段。该方法灵敏、快速、特异性高．可用于羊附红细胞体病的早期快速诊断和流行病学调查。  相似文献   

Variation of pesticide concentration in sheep dips operated according to traditional and revised methods     

Sherwood N  Watts T  Allan C  Chandler D 《Australian veterinary journal》1999,77(6):384-387

OBJECTIVE: To quantify stripping in traditional dipping operations and to revise dipping methods, based on prediction of stripping so that a more stable concentration of pesticide in the dipwash is achieved. DESIGN AND METHODS: Plunge and shower dips were operated sequentially according to traditional and revised dipping instructions. Dips were operated by continuous and intermittent replenishment. Samples of mixed dipwash were collected periodically and assayed for pesticide (diazinon) concentration. RESULTS: Diagrammatic representations of pesticide concentration versus number of sheep dipped indicated traditional dipping leads to wide variations in the concentration of pesticide in dipwash during dipping. Intermittent replenishment led to a 'saw-tooth' pattern in the pesticide concentration. Traditional continuous replenishment (using the starting concentration of pesticide) indicated both the rate and extent of stripping was higher in shower dipping. If sufficient sheep were dipped, equilibrium was reached between the rate of pesticide replenishment and removal. An alternative method of dip operation by continuous replenishment, using a low starting concentration of pesticide and a replenishment concentration high enough to offset the pesticide loss through stripping resulted in a more stable concentration of pesticide in the dip. CONCLUSION: Revision of dipping instructions can lead to exposure of sheep to stable concentrations of stripping pesticide during dipping.  相似文献   

阿维菌素长效注射液(油悬剂)对绵羊痒螨的疗效试验     

张亚峰  王玉万  汪明  潘贞德  潘保良 《畜牧与兽医》2006,38(1):17-19

为了研究阿维菌素长效注射液(油悬剂)对绵羊痒螨疗效,将60只自然感染痒螨绵羊随机分为3组,每组20只。第1组每千克体重颈部皮下注射1 mg的阿维菌素油悬剂,第2组注射0.2 mg的阿维菌素普通注射液,第3组为不给药对照组。在给药后第7、14、21、28、35、42、49、56、63、70天对所有绵羊进行螨虫检查和计数,每天观察病变。结果表明:给自然感染痒螨的绵羊皮下注射1 mg/kg的阿维菌素长效注射液(油悬剂)可在给药后7 d内将痒螨完全杀灭,在给药后63 d内防止绵羊被痒螨再感染,其持效期远长于阿维菌素普通注射液(约为14 d)。  相似文献   

Epidemiological Observations in a Corriedale Flock affected by Brucella ovis     

Robles  C.A.  Uzal  F.A.  Olaechea  F.V.  Low  C. 《Veterinary research communications》1998,22(7):435-443

Brucellosis in sheep, caused by Brucella ovis, is primarily a chronic infectious disease of rams with epididymitis as its most characteristic lesion. Six hundred rams from an infected farm were clinically and serologically examined once a year, over a 3-year period. An increase from 2.1% to 6.3% in the prevalence of animals serologically positive to B. ovis occurred over the 3 years. However, the prevalence of rams with lesions in the reproductive tract declined from 14.2% to 6.5% in the third year following one year of strict culling of clinically affected and rams that were serologically positive for B. ovis. Clinical lesions found in the 179 affected rams fell into two main categories: rams with epididymitis and rams with affected lymph nodes. These results suggest that the prevalence of the disease relates mainly to the sexual activity of the animal and not to age in itself. A single cull based on the results of clinical examination and serological test results was unable to decrease the prevalence of B. ovis in an extensive Corriedale sheep flock.  相似文献   

Using remote sensing to predict outbreaks of Oestrus ovis in Namibia     

S. Flasse  C. Walker  H. Biggs  P. Stephenson  P. Hutchinson 《Preventive veterinary medicine》1998,33(1-4):31-38

The Directorate of Veterinary Services of the Ministry of Agriculture, Water and Rural Development of Namibia issues warnings to farmers in the south of the country about the likelihood of infestation of small-stock by the nasal bot fly, Oestrus ovis. Farmers can then treat their stock at the most appropriate time. The O. ovis puparia develop at shallow depths in the soil and the timing of emergence is directly dependent on climatic conditions, specifically the number of degree-days above a particular threshold soil temperature. Based on temperature measurements from only a few stations scattered throughout the country, the veterinary department warnings lack precision in space and time. This paper presents an attempt to support the programme of warnings with accumulated temperature information from Meteosat satellite images, in order to strengthen predictions of the time of emergence in specific places, and to improve the precision and reliability of warnings given to farmers.  相似文献   

吸吮绵羊附红细胞体感染的母羊乳汁的羔羊保护力观察     

高光平  毕可东  张剑  王晓丽 《河南农业科学》2006,(3):112-114

给初生并吸吮感染绵羊附红细胞体(E.ovis)母羊乳汁的羔羊注射含有大量E.ovis的肝素抗凝的血液,直到羔羊断奶,结果羔羊体内的E.ovis或被杀灭或未出现明显的感染。试验证明,除了乳汁外羔羊不能获得对E.ovis感染的抵抗力。  相似文献   

培植羊黄的扫描电镜观察     

王春璈  郭延奎  阎青  朱连德 《山东农业大学学报(自然科学版)》1991,(1)

对培植羊黄进行了扫描电镜观察,并与培植牛黄,天然牛黄进行比较。三种黄的超微结构相似,分层明显,都具有丝网状结构,黄的基质部分为硫酸化多糖粘蛋白所构成。  相似文献   

人工培植羊黄的研究     

王春ao  胡孝忠 《畜牧兽医学报》1991,22(3):257-262

对70头山羊、50头绵羊和5头奶山羊进行了人工培植羊黄研究。在植黄后一年,对42头山羊,10头绵羊和1头奶山羊取黄,在羊的胆囊内形成了羊黄。对11个羊黄样品进行了胆色素、总胆固醇和总胆酸等项目测定,结果表明与天然牛黄主要成分相同,但含量有所不同。对5个羊黄样品进行了超微结构观察,结果与天然牛黄一致。 对人工培植羊黄羊的胆囊胆汁进行了测定,β-葡萄糖醛酸苷酶活性(以下简称β-G酶活性)和粘蛋白含量明显升高。因接种的大肠杆菌血清型不同,β-G酶活性也呈现不同程度的变化。对培植羊黄羊的胆囊进行了组织学检查,胆囊壁粘膜上皮柱细胞间的杯状细胞及固有膜中的粘液腺大量增生,是胆汁中多糖粘蛋白增高的组织学基础。 对培植羊黄进行了药理学试验,其结果与天然牛黄完全一样。  相似文献   

猪带绦虫不同阶段及羊囊尾蚴蛋白质分析     

魏竹波  高荣  沈斌  孟民杰  王克非  刘世贵  朱锦  付玉梅  卓麟 《中国兽医杂志》2001,37(8):11-13

利用浓度梯度SDS－聚丙烯酰胺凝胶电泳对猪囊尾蚴的囊液和虫体，猪带绦虫成虫的头颈节、成节、孕节，猪带绦虫的虫卵以及羊囊尾蚴的蛋白成分进行了对比分析。结果发现：囊液、虫体、头颈节、成虫、孕节、虫卵、羊囊尾蚴分别有35条（15．0－150．4kd）、44条（15．0－133．7kd)、22条（13．3－163．9kd)、19条（13．3－90．1kd)、19条（13．3－90．1kd)、30条（13．3－99．4）和38条（15．0－133．4kd)蛋白带；其中15．0kd和35．5kd两条共同抗原蛋白带，在猪带绦虫各阶段和羊囊尾蚴中含量最大的蛋白带同时也是阶段性的共同抗原蛋白带。这些结果为下一步的保护性抗原筛选奠定了基础。  相似文献   

Further data to the aetiology, pathogenesis and therapy of infectious bovine keratoconjunctivitis     

Attila Nagy  mile Vandersmissen  Pl Kapp 《Comparative immunology, microbiology and infectious diseases》1989,12(4):115-127

Out of a total of 224 bovine eye secretions, 126 Moraxella bovis and 64 Neisseria ovis strains were isolated. The pathogenesis and histological lesions caused by Neisseria ovis have been studied on the eyes of three calves naturally affected with IBK, using electron microscopy.

Neisseria ovis caused in 1–12 weeks old calves acute, transient and mostly benign serous conjunctivitis with only slight affection of the cornea. More rarely erosions and even ulceration of the cornea have been observed.

Moraxella bovis and Neisseria ovis strains proved nearly unanimously sensitive in vitro to chloramphenicol, neomycin, oxytetracyclin, nitrofurantoin, erythromycin and cefoperazone. Other antibiotics and chemotherapeutics inhibited the growth of these agents only partly or were ineffective.

Experimental therapy has been carried out using a single i.m. injection of Terramycin/LA inj. (Pfizer) in a dose of 20 mg/kg body mass, repeated if necessary after 72–96 h. This formulation proved more effective and practical than treatments used earlier.  相似文献